Molecular cytogenetic identification of newly synthetic Triticum kiharae with high resistance to stripe rust

Xiaojuan Liu,Minghu Zhang,Xin Liu,Hongyu Li,Ming Hao,Shunzong Ning,Zhongwei Yuan,Dengcai Liu,Bihua Wu,Xuejiao Chen,Wenjie Chen,Lianquan Zhang

Genetic Resources and Crop Evolution

August 2018, Volume 65, Issue 6, pp 1725–1732

Abstract

Six new amphiploids,Triticum kiharaeDorof. et Migusch. (2n = 6x = 42, AtAtGGDD), are described in this study. They were developed by the chromosome doubling of F1hybrid crosses betweenTriticum timopheeviiZhuk. (AtAtGG) with high resistance to stripe rust andAegilops tauschiiCosson (DD) by colchicine treatment. These amphiploids showed a high level of fertility of 68–80% and exhibited relatively normal chromosome pairing in meiotic metaphase I. Individual chromosomes ofT. kiharaecould be identified by multicolor fluorescence in situ hybridization using the combination of oligonucleotides probes Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-pTa71-2.T. kiharaeexhibited high resistance to predominant stripe rust races CYR34, CYR31, CYR32, CYR33, and SY11-4 both during the seedling and adult stages. However, high molecular weight glutenin subunits fromAe. tauschiiparents were only partially expressed in theT. kiharaebackground. TheseT. kiharaelines provide novel materials to widen the genetic diversity of the common wheat gene pool.